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The 460 nm measurement was utilized to report reduction of the flavin, while the 540 nm observation was made to track the formation of broad thiolate-FAD charge transfer absorption ( SmTrx titration to SmTGR U597S To demonstrate the function of the 596597 disulfide the reductive half-reaction of SmTGR U597S variant in the presence of the oxidant substrate Trx was observed
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