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Site-directed mutagenesis was performed using a QuikChange kit (Agilent) and a primer pair (Supplementary Table 3) to incorporate an upstream ribosomal binding site
Abstract Animals rely on nervous systems to cope with environmental variability, whereas plants are characterized by lack of nervous system but still have evolved systemic communication systems through signaling molecules that trigger long-distance defense signaling events when encountered with environmental challenges
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Blots were normalized against -actin levels
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