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Preparation Condition 4 (EDTA 4 o C deproteinization): Storage at 80 o C prevents enzymatic degradation of GSH We included EDTA-treated plasma stored at 4 o C (Preparation Condition 4) as a positive control for enzymatic processes that may lead to degradation of GSH during sample storage

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Aim for 1.2 to 1.6 grams of protein per kilogram of body weight per day, with a minimum of 30 grams per meal to stimulate muscle protein synthesis