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Approximately 1 g of peptide was injected and separated via liquid chromatography using a VanquishNeo UHPLC (Thermo, VN-S10-A-01) in Trap-and-Elute configuration using a PepMap Neo (5 M 300 M 5 mm) trap (Thermo, 174500) and an Aurora Frontier 60 cm 75 m C18 analytical column (IonOpticks, AUR3-60075C18) operating at a flow rate of 300 nl min 1 at a column temperature of 50 C
Alexis F, Pridgen E, Molnar LK, Farokhzad OC (2008) Factors affecting the clearance and biodistribution of polymeric nanoparticles
But this cycle can slow down, and dead cells may accumulate on the surface