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Mouse anti-GLP-1R monoclonal antibody was from DSHB (Iowa, IA, USA)

Jung SY,Eberlin LS,Johnson JR,Kittles RA,Ballester LY,Parsawar K,Siddiqui MM,Gao J,Langer Gramer A,Bollag RJ,Terris MK,Lotan Y,Creighton CJ,Lerner SP,Sreekumar A,Kaipparettu BA,Putluri N

For an IM injection, which goes directly into the muscle, youll want to hold the syringe like a dart at a 90-degree angle to your skin

Peripheral blood mononuclear cells were isolated by Ficoll density gradient centrifugation and monocytes were isolated by plastic adherence by culturing 1 10 6 cells/mL for 2 h in serum-free media at 37 C [20]