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We drew a standard curve using the gradient dilution of the standard product as a template, added the sample, antibody and HRP aggregation reagent, and put it in a 37C water bath, then washed it five times with a washing solution, we added the chromogenic solution and reacted for 10 min, then detected the absorbance of each hole at a wavelength of 450 nm

While Luxol fast blue (LFB) staining initially revealed a similar degree of demyelination between the two strains, examination at later time-points revealed a more efficient remyelination in DA Gsta4 compared to in DA Wt (Fig

Watch for additive serotonin effects if combined with behaviour drugs (see notes)

Biochemistry 40 , 1318813200 (2001)

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