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From a medical standpoint, the most meaningful changes happen over time
Singularizing and counting cells from COs To singularize cells from COs, the COs were first washed twice with PBS and then incubated with 2 mL of Accutase TM (AT-104, Innovative Cell Technology Inc., San Diego, CA, USA) as described in detail in Duong et al
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Food Chem 338:127991 Benkov E, Michniewicz M, Sauer M, Teichmann T, Seifertov D, Jrgens G, Friml J (2003) Local, efflux-dependent auxin gradients as a common module for plant organ formation
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