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Briefly, cells stained with the indicated antibodies ( Supplementary Table 1 ) and then fixed and permeabilized using a Cytofix/Cytoperm kit (BD Bioscience) or a Transcription Factor Buffer Set (BD Biosciences) and stained with the corresponding antibodies (IFN-, IL-21, IL-10, FoxP3, TCF1, and Eomes)

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