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Oxalate induces type II epithelial to mesenchymal transition (EMT) in inner medullary collecting duct cells (IMCD) in vitro and stimulate the expression of osteogenic and fibrotic markers in kidney medulla in vivo

To verify this, we firstly compared the growth of D39 with no methionine and 100 g/mL glutamine (M0Q100), no methionine and 1,000 g/mL glutamine (M0Q1000), 200 g/mL methionine and 100 g/mL glutamine (M200Q100), and 200 g/mL methionine and 1,000 g/mL glutamine (M200Q1000)

If you already used sterile water, draw all remaining doses into capped syringes, refrigerate, and use within 48 hours

This approach targets 8 specific sites, significantly reducing DAR heterogeneity compared to lysine conjugation

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