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Allowing both the peptide vial and the bacteriostatic water to equilibrate to room temperature before beginning resolves a significant percentage of cases without any other intervention

A comparison of the co-crystal structures of DPP-4 with saxagliptin (PDB ID 3bjm, Figure 4) and DPP-4 with its substrate, Diprotin A (Ile-Pro-Ile), (PDB ID 1nu8, Figure 5) reveals that saxagliptin acts by occluding the DPP-4 active site and prevents binding of incretin hormones

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