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Mammalian cell culture HeLa cells were acquired directly from ATCC and HEK293FT from Invitrogen

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Reduced expression of tyrosine hydroxylase and TPH, biochemical markers of neuronal integrity, was observed after mephedrone administration, along with downregulation of dopamine D2 receptors in the striatum, indicating the presence of damaged nerve endings 48,49

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Preclinical evidence suggests that -TOH administration attenuates this cascade by targeting protein kinase (PKC), a negative regulator of GS expression [118]