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Wegovy acts like a GLP-1 hormone, which works in the brain to regulate a persons appetite

Immunobl o tting RAW264.7 cells were cultured in 12-well plates at 2 x 10 6 cells per well/experimental condition

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Following this, differentiated HepaRG cells were seeded in the opposite hepatic chamber at a density of 1.85 10 5 cells/cm 2 (3 10 5 cells in total) in hepatic thawing and seeding medium containing Williams Medium E, 5% (v/v) FCS, 4% hepatocyte thawing and plating supplements CM3000 (GIBCO), 1 M dexamethasone (GIBCO), 2 mM L-glutamine, 5 g/mL insulin, 5 M hydrocortisone-hemisuccinate and AAS

These ticks are tiny, especially in their nymph stage when they are about the size of a poppy seed