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This is not inherently problematic since someone has to do the initial research

For transfection, the cells were cultured until 70% confluency and transfected with the pCMV-2B FLAG-GST expression vector using Lipofectamine 3000 reagent in Opti-MEM (Gibco) medium according to the manufacturers protocol (Invitrogen)

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Discontinuation may be warranted if allergic reactions occur, if side effects persist despite slower dosing, or if baseline conditions (acute kidney injury, active infection) change

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