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The resulting supernatant is ready for cleanup
Nonspecific binding was determined by incubating tissue sections in the incubation solution containing additionally 100 nM unlabeled GLP1(736)amide (Bachem, Bubendorf, Switzerland) that, at this concentration, completely and specifically displaces 125 I- GLP1(736)amide at the receptors
reducing the risk of cardiovascular disease
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& Koh, G
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