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The plates were sealed with AeraSeal films (Merck, A9224) to allow gas exchange in cultures and incubated at 30 C, 225 rpm and 80% relative humidity in a humidity shaker (Minitron, INFORS)
Muscle damage and muscle remodeling: no pain, no gain
Results build gradually over a 12-week cycle
The general rule of thumb is that 1,000 mg is a good middle ground that works for most people looking to lose fat, especially when paired with regular exercise
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