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The resulting glutamate, glycine, and cysteine will then be absorbed into cells through an amino acid transporter (Fig
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Subsequently, the cells were treated with PBS, Ru(II)-OH, NP2, or NP4 at various final concentrations ranging from 0.025 M to 15 M for 48 h
After 24 h transfection, cells were resuspended with ice-cold GST-lysis buffer (20 mM Tris-HCl, pH 8.0, 1% Triton X-100 and 10% glycerol in PBS) supplemented with a protease inhibitor cocktail (Roche) and 50 M Palmostatin B (for SwissKASH-derived samples), and passed 10 times through a 25 G needle
NAC may also reduce the formation of glutamate, an excitatory neurotransmitter that kills brain cells in excess
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