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[13] The first reaction of the carnitine shuttle is a two-step process catalyzed by a family of isozymes of acyl-CoA synthetase that are found in the outer mitochondrial membrane , where they promote the activation of fatty acids by forming a thioester bond between the fatty acid carboxyl group and the thiol group of coenzyme A to yield a fatty acylCoA
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Thereby, PI3K and AKT transcription was downregulated 1.7- and 2-fold, respectively, whereas PGC-1 transcripts were increased 7-fold and AKT phosphorylation was reduced by 3550%
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NAD+ is essential for cellular respiration, with roles in glycolysis, the Krebs cycle, and the electron transport chain
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