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The dissociated single cells were seeded on AggreWell TM 800 plates (Stemcell Technologies, USA #34850) containing 300 microwells per well, pretreated with AggreWell rinsing solution (Stemcell Technologies, USA #07010), according to the users manual
Giri et al., 2022) as well as mutation within the host (Valesano et al., 2021
To obtain ratiometric images, the masks were applied to the background-subtracted images and the FP channel was divided by the SiR channel in a pixel-by-pixel manner within the ROIs
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doi: 10.5281/zenodo.14635348
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