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Siegel, 1961): Absorption tests with food, using a flushing vitamin B12 injection to estimate absorption, indicate a reduction of the passive absorption by about 40% when supplements are taken with food rather than when fasting
LCMS metabolomics analysis Sample preparation HCT116s cells (1 10 5 ) treated with or without 5 M 5FU were cultured in DMEM for 24 h and 72 h or supplemented with media containing uniformly labelled 13 C 6 -glucose (25 mM) for 6 h before sampling
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Yet another study found that a vegan diet with low protein intake results in significantly lower blood GSH levels compared to omnivores and lacto-ovo-vegetarians [187]
Best practice is to prepare single-use aliquots before freezing to minimize freeze-thaw events per volume
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