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An opened vial is good for 28 days from the first puncture
A standard expression induction protocol was based on shifting log-phase cultures (A600 = ~0.6) from 37 C and adding IPTG (1 mM)
Science 323:12051208 Vance C, Rogelj B, Hortobgyi T et al (2009) Mutations in FUS, an RNA processing protein, cause familial amyotrophic lateral sclerosis type 6
Tamir S, Izrael S, Vaya J: The effect of oxidative stress on ERalpha and ERbeta expression
Obstructive sleep apnea, obesity hypoventilation syndrome, and asthma are prevalent in this population
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