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Because nicotinamide would otherwise re-enter the NAD+ salvage pathway through NAMPT and NMN, NNMT activity sits at a junction between cellular methylation capacity and NAD+ regeneration, which is why the enzyme became a target after Kraus and colleagues (Nature, 2014) reported the consequences of antisense Nnmt knockdown in white adipose tissue and liver
These are preclinical findings, not human clinical proofs
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This increases its bioavailability and ensures sustained interaction with target cells, optimizing its effects on muscle growth, body weight loss, and overall tissue regeneration
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