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Angiopoietin-2 regulates gene expression in TIE2-expressing monocytes and augments their inherent proangiogenic functions

If the picture supports a different intervention, that is what we run

At the end of the experiment, the cells were counterstained with NucBlue (Invitrogen), according to the manufacturers instructions, and were mounted with ProLong Diamond Antifade Mountant (Invitrogen) for microscopic fluorescence analysis under an Olympus BX50 microscope (Olympus, Tokyo, Japan)

Ledgers are optional (for auditing/anchoring), not required

Always inspect the solution before each use for cloudiness, particles, or discoloration