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Liang, T
370 PolimeniG.EspositoE.BevelacquaV.GuarneriC.CuzzocreaS
The samples were diluted in RIPA, mixed with Laemmli buffer (Bio-Rad, Hercules, CA), and then denatured at 95C for 5 min
It requires at least 2 hours , but can be more or less
The Absorption Challenge: Why Bioavailability Is Key The most important question to ask is not just "how much am I taking," but "how much is my body actually absorbing?" This is the core concept of bioavailability, which refers to the proportion of a substance that enters the circulation and is able to have an active effect
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