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Harlan Krumholz: Yeah, Im really excited about having Eric on the program, someone I admire a lot and has made such great contributions

Real-time quantitative polymerase chain reaction Total RNA was extracted from cells using the Trizol reagent (Takara Bio Inc, Japan) according to the manufacturers instructions

DNA was isolated using the Qiagen Plasmid Mini kit or the Qiagen Plasmid Plus Midi kit

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