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For each assay, cells were plated in duplicate, with one plate containing CellTox Green Dye added according to the Express, No-Step Addition at Seeding protocol described in the CellTox Green Cytotoxicity Assay Technical Manual #TM375 (10l of CellTox Green Dye per 5ml of cells)
Three methyl groups of the lactone ring interact with a hydrophobic surface formed by A2058, A2059, and U2611
Description Buy Epitalon research-grade synthetic tetrapeptide (Ala-Glu-Asp-Gly), 99% purity verified by independent HPLC and mass spectrometry, US-manufactured with a batch-specific Certificate of Analysis included in every order
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